Wnt-surrogate

货号(Catalog Number):

WSE-H100

价格:

¥ 2000

  • 规格
    • 100μg
    • 1mg
数量
-
+

库存

200

隐藏域元素占位

  • 产品描述
  • 产品数据

    产品描述(DESCRIPTION)

    种属

    Species

    Human

    分子别名

    Other name

    /

    Accession

    /

    标签

    Tag

    Human IgG1 FC

    表达系统

    system

    Human embryonic kidney 293 cells, HEK293

    表达区间

    Source

    /

    质量属性(SPECIFICATIONS)

    分子量

    MW

    The protein migrates as 140kDa under non reducing (NR) condition (SDS-PAGE) 

    活性

    Activity

    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
    Optimal concentrations  should be determined by each laboratory for each application.

    内毒素

    Endotoxin

    <1EU/UG

    纯度

    Purity

    >95% as determined by SDS-PAGE.

    制剂

    Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
    0.0%1 Tween 80 were added as protectant before lyophilization

    保存与使用(PREPARATION AND STORAGE)

    溶解方法

    Reconstitution

    Reconstitute at 100 μg/mL in sterile PBS.

    运输

    Shipping

    The product is shipped with Blue ice.

    存储

    Storage

    For long term storage, the product should be stored at lyophilized state at -20°C,avoid
    repeated freezethaw cycles.

    稳定性

    Stability

    >12 months, under -20°C conditions before reconstitution.
    >6 months, -80 °C under sterile conditions after reconstitution

    背景介绍(BACKGROUND)

    Wnt surrogate offers a novel solution to overcome the solubility challenge, which a major obstacle of working with Wnts. The Wnt surrogate is an engineered Fc fusion protein, which bridges the FZD and LRP5/6 receptors to activate Wnt signaling pathways with improved solubility and at much lower concentrations required compared to commercially available recombinant Wnt3a proteins or Wnt-conditioned media. The Wnt surrogate is composed of the LRP-binding domain of the extracellular Wnt modulator Dickkopf (DKK), linked to a designed ankyrin repeat protein (DARPin)—a selective, high affinity binder of Frizzled receptors.  More specifically, this engineered Wnt mimetic interacts with FZD1, FZD2, FZD5, FZD7, and FZD8.

  • 4%-20% SDS-PAGE Result Wnt-surrogate, 2ug non-reducing

    Wnt-surrogate induce Topflash reporter activity in HEK293T human embryonic kidney cells.  EC50:20ng/ml

     

     

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