The protein migrates as 140kDa under non reducing (NR) condition (SDS-PAGE)
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Optimal concentrations should be determined by each laboratory for each application.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped with Blue ice.
For long term storage, the product should be stored at lyophilized state at -20°C，avoid
repeated freezethaw cycles.
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution
Wnt surrogate offers a novel solution to overcome the solubility challenge, which a major obstacle of working with Wnts. The Wnt surrogate is an engineered Fc fusion protein, which bridges the FZD and LRP5/6 receptors to activate Wnt signaling pathways with improved solubility and at much lower concentrations required compared to commercially available recombinant Wnt3a proteins or Wnt-conditioned media. The Wnt surrogate is composed of the LRP-binding domain of the extracellular Wnt modulator Dickkopf (DKK), linked to a designed ankyrin repeat protein (DARPin)—a selective, high affinity binder of Frizzled receptors. More specifically, this engineered Wnt mimetic interacts with FZD1, FZD2, FZD5, FZD7, and FZD8.
4%-20% SDS-PAGE Result Wnt-surrogate, 2ug non-reducing
Wnt-surrogate induce Topflash reporter activity in HEK293T human embryonic kidney cells. EC50:20ng/ml