Recombinant Human BAFF

货号(Catalog Number):

RP8064

价格:

¥ 2000

  • 规格
    • 100μg
数量
-
+

库存

1000

隐藏域元素占位

  • 产品描述
  • 产品数据

    产品描述(DESCRIPTION)

    Source 

    Human embryonic kidney 293 cells, HEK293
    Ala134-Leu285, with a FC  fusion tag  at the N-terminal 
    Accession # AAH20674.1

    Species

    Human

    Predicted Molecular Mass

    45 kDa

    质量属性(SPECIFICATIONS)

    SDS­PAGE 

    The protein migrates as 55  kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

    Purity 

    >95% as determined by SDS-PAGE.

    Formulation 

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 
    0.01%   Tween 80 were added as protectant before lyophilization

    保存与使用(PREPARATION AND STORAGE)

    Shipping 

    The product is shipped with Blue ice.

    Reconstitution 

    Reconstitute at 100 μg/mL in sterile PBS.

    Storage

    For long term storage, the product should be stored at  lyophilized state at -20°C,avoid repeated freeze­thaw cycles.

    Stability

    >12 months, under -20°C conditions  before reconstitution.
    >6 months,  -80 °C under sterile conditions after reconstitution.

    背景介绍(BACKGROUND)

    B-cell activating factor (BAFF) is also known as tumor necrosis factor ligand superfamily member 13B , TNFSF13B, BAFF, B Lymphocyte Stimulator (BLyS) , cluster of differentiation 257 (CD257), DTL, TNF- and APOL-related leukocyte expressed ligand (TALL-1), THANK, TNFSF20, ZTNF4, and is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling through BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

  •  

    SDS-PAGE

    4%-20% SDS-PAGE Result Recombinant BAFF, 2ug reducing