Recombinant R-Spondin 2

货号(Catalog Number):

RP8060

价格:

¥ 2000

  • 规格
    • 100μg
数量
-
+

库存

10000

隐藏域元素占位

  • 产品描述
  • 产品数据

    产品描述(DESCRIPTION)

    Source 

    Human embryonic kidney 293 cells, HEK293
    Gln22-Gly205, with a FC tag  at the C-terminal
    NP_848660.2

    Species

    Human

    质量属性(SPECIFICATIONS)

    SDS­PAGE 

    The protein migrates as 70 KDa under reducing (R) condition (SDS-PAGE) .

    Purity 

    >95% as determined by SDS-PAGE.

    Formulation 

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 
    0.01% Tween 80 were added as protectant before lyophilization

    保存与使用(PREPARATION AND STORAGE)

    Shipping 

    The product is shipped with dry ice.

    Reconstitution 

    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    Storage

    For long term storage, the product should be stored at  lyophilized state at -20°C,avoid repeated freeze­thaw cycles.

    Stability

    >12 months, under -20°C conditions  before reconstitution.
    >6 months,  -80 °C under sterile conditions after reconstitution.

    背景介绍(BACKGROUND)

    Roof plate-specific Spondin 2 isoform 1 (R‑Spondin 2, RSPO2), also known as cysteine‑rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family (1‑3). The four R‑Spondins regulate Wnt/ beta -catenin signaling and overlap in expression and function (1‑3). Like other R‑Spondins, RSPO2 contains two adjacent cysteine‑rich furin-like domains (aa 90‑134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207‑243). The basic region binds heparin and mediates cell surface retention and extracellular matrix attachment while the furin‑like domains are required for Wnt/ beta -catenin signaling (1, 3, 4). RSPO2 contains one potential N‑glycosylation site. Mature human RSPO2 shares 97‑98% aa identity with mouse, rat, equine, canine and bovine RSPO2 and ~40% aa identity with RSPO1, RSPO3 and RSPO4. Of the three reported splice isoforms of human R‑Spondin 2, isoform 2 lacks residues 1 ‑ 67 of isoform 1, while isoform 3 has a glycine substitution for residues 32‑95 of isoform 1 (5). Human RSPO2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is down‑regulated in tumors of these tissues (1). In the embryonic mouse, RSPO2 expression is concentrated in the apical epidermal ridge, hippocampus, and developing muscle, teeth and bones (1, 6). Deletion of RSPO2 results in down‑regulation of Wnt activity in these areas, malformations of the facial skeleton and limbs, and respiratory failure at birth (7‑9). RSPO2 is an extracellular potentiator of Wnt/ beta -catenin signaling (3, 4). It functions at least in part by binding LRP‑6, stimulating its long-term phosphorylation and down‑regulating its internalization (3, 4). RSPO proteins, especially RSPO2 and RSPO3, also antagonize DKK1 activity by interfering with DKK1‑mediated LRP‑6 and Kremen association (10)

  •  

    4%-20% SDS-PAGE Result Recombinant R-Spondin-2, 2ug reducing

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