- 商品名称: Recombinant Human Heregulinβ-1
- 商品编号: NRG-902
- 物种: Human
- 表达系统: HEK293
- 标签: HIS
- 纯度: >95%
- 活性: 细胞活性
Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine, Receptor Inducing Activity), glial growth factor
Measured by its ability to bind HER3 by elisa method
<1.0 EU per 1 μg of the protein by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped with blue ice.
For long term storage, the product should be stored at lyophilized state at -20°C，avoid repeated freezethaw cycles.
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution.
Neuregulin/Heregulin is a family of structurally-related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1's biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).
4%-20% SDS-PAGE Result Recombinant Human Heregulinβ-1, 2ug reducing