Recombinant Human Heregulinβ-1

货号(Catalog Number):

NRG-902

价格:

¥ 2000

  • 规格
    • 100μg
数量
-
+

库存

100

隐藏域元素占位

  • 产品描述
  • 产品数据

    产品描述(DESCRIPTION)

    种属 
    Species

    Human

    分子别名
    Other name

    Neuregulin1 (NRG1-β1), Neu differentiation factor (rat), HRG, HRG1-β1, Breast cancer cell differentiation factor p45, ARIA (Acetylcholine, Receptor Inducing Activity), glial growth factor

    Accession

    Q02297

    标签
    Tag

    HIS

    表达系统
    system

    HEK293

    表达区间
    Source

    Met1-Lys2468

    质量属性(SPECIFICATIONS)

    分子量

    MW

    25 kd

    活性
    Activity

    Measured by its ability to bind HER3 by elisa method

    内毒素
    Endotoxin

    <1.0 EU per 1 μg of the protein by the LAL method.

    纯度
    Purity

    >95% as determined by SDS-PAGE.

    制剂
    Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
    0.0%1 Tween 80 were added as protectant before lyophilization

    保存与使用(PREPARATION AND STORAGE)

    溶解方法

    Reconstitution

    Reconstitute at 100 μg/mL in sterile PBS.

    运输
    Shipping

    The product is shipped with blue ice.

    存储
    Storage

    For long term storage, the product should be stored at  lyophilized state at -20°C,avoid repeated freeze­thaw cycles.

    稳定性
    Stability

    >12 months, under -20°C conditions  before reconstitution.
    >6 months,  -80 °C under sterile conditions after reconstitution.

    背景介绍(BACKGROUND)

    Neuregulin/Heregulin is a family of structurally-related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation. Although HRG1-β1's biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells, which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF).

  •  

    4%-20% SDS-PAGE Result Recombinant Human Heregulinβ-1, 2ug reducing