Recombinant Human R-Spondin-1

货号(Catalog Number):

RP8029-100

价格:

¥ 2000

  • 规格
    • 100μg
    • 1mg
数量
-
+

库存

200

隐藏域元素占位

  • 产品描述
  • 产品数据

    产品描述(DESCRIPTION)

    种属

    Species

    Human

    分子别名

    Other name

    /

    Accession

    UniProt# Q2MKA7

    标签

    Tag

    HIS

    表达系统

    system

    Human embryonic kidney 293 cells, HEK293

    表达区间

    Source

    /

    质量属性(SPECIFICATIONS)

    分子量

    MW

    The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

    活性

    Activity

    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
    Optimal concentrations  should be determined by each laboratory for each application.

    内毒素

    Endotoxin

    <1EU/UG

    纯度

    Purity

    >95% as determined by SDS-PAGE.

    制剂

    Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
    0.0%1 Tween 80 were added as protectant before lyophilization

    保存与使用(PREPARATION AND STORAGE)

    溶解方法

    Reconstitution

    Reconstitute at 100 μg/mL in sterile PBS.

    运输

    Shipping

     

    The product is shipped with Blue ice.

    存储

    Storage

    For long term storage, the product should be stored at lyophilized state at -20°C,avoid
    repeated freezethaw cycles.

    稳定性

    Stability  

    >12 months, under -20°C conditions before reconstitution.
    >6 months, -80 °C under sterile conditions after reconstitution

    背景介绍(BACKGROUND)

    R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells

  • 4%-20% SDS-PAGE Result Recombinant Noggin, 2ug reducing &Non-reducing

    Wnt-surrogate induce Topflash reporter activity in HEK293T human embryonic kidney cells.  EC50:20ng/ml

     

     

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