The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Optimal concentrations should be determined by each laboratory for each application.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and
0.0%1 Tween 80 were added as protectant before lyophilization
保存与使用（PREPARATION AND STORAGE）
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped with Blue ice.
For long term storage, the product should be stored at lyophilized state at -20°C，avoid
repeated freezethaw cycles.
>12 months, under -20°C conditions before reconstitution.
>6 months, -80 °C under sterile conditions after reconstitution
R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R-Spondin-1 activates β-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK1 for binding to the WNT coreceptors, Kremen and LRP6, which reduces DKK1-mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells
4%-20% SDS-PAGE Result Recombinant Noggin, 2ug reducing &Non-reducing
Wnt-surrogate induce Topflash reporter activity in HEK293T human embryonic kidney cells. EC50:20ng/ml