Human embryonic kidney 293 cells, HEK293
Arg328-Phe528, HIS tag
Predicted Molecular Mass
The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Measured by its ability to bind Ace2 by elisa method
<1 EU per 1 μg of the protein by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from a 0.2 μm filtered solution in PBS.
- PREPARATION AND STORAGE
The product is shipped with dry ice.
For long term storage, the product should be stored at lyophilized state at -80°C，avoid repeated freezethaw cycles.
>6 months,under -80°C conditions
Spike protein S1:attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Can be alternatively processed by host furin (PubMed:32362314). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes