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规格单价
100ug2300

Recombinant Human R-Spondin-1

Catalog number: RP8019-100

DESCRIPTION
Source

Human embryonic kidney 293 cells, HEK293
UniProtKB# Q2MKA7,with a stable fusion tag at the C-terminal

Species

Human

Predicted Molecular Mass

    

SPECIFICATIONS
SDS-PAGE

The protein migrates as100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
Optimal concentrations should be determined by each laboratory for each application.

Endotoxin Level

<1 EU per 1 μg of the protein by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

PREPARATION AND STORAGE
Shipping

The product is shipped with dry ice.

Storage

For long term storage, the product should be stored at lyophilized state at -80°C,avoid repeated freeze­thaw cycles.

Stability

>6 months,under -80°C conditions

DATA
BACKGROUND
R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R­-Spondin-1 activates β­-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK­1 for binding to the WNT co­receptors, Kremen and LRP­6, which reduces DKK­1-­mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells

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