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| 规格 | 单价 |
|---|---|
| 100ug | 2300 |
Recombinant Human R-Spondin-1
Catalog number: RP8019-100
- DESCRIPTION
-
Source
Human embryonic kidney 293 cells, HEK293 UniProtKB# Q2MKA7，with a stable fusion tag at the C-terminal
SpeciesHuman
Predicted Molecular Mass - SPECIFICATIONS
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SDS-PAGE
The protein migrates as100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
ActivityMeasured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Optimal concentrations should be determined by each laboratory for each application.
Endotoxin Level<1 EU per 1 μg of the protein by the LAL method.
Purity>95% as determined by SDS-PAGE.
FormulationLyophilized from a 0.2 μm filtered solution in PBS.
- PREPARATION AND STORAGE
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Shipping
The product is shipped with dry ice.
StorageFor long term storage, the product should be stored at lyophilized state at -80°C，avoid repeated freezethaw cycles.
Stability>6 months,under -80°C conditions
- DATA
-
- BACKGROUND
- R-Spondin-1 (RSPO1) is the prototype member of the R-Spondin (RSPO) protein subfamily of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins (Chen et al.; Kamata et al.; Kazanskaya et al.; Kim et al.). Although unable to initialize signaling, RSPO family members are potent enhancers of WNT signaling (Cruciat & Niehrs; de Lau et al.; Kamata et al.; Kazanskaya et al.). They are characterized by a TSR-1 domain, a carboxy-terminal region with positively charged amino acids, and two N-terminal furin-like cysteine-rich repeats (Glinka et al.; Kazanskaya et al.). R-Spondin-1 activates β-catenin signaling via the WNT signaling cascade and by indirectly increasing low-density lipoprotein receptor-related protein (LRP6) on the cell surface. It does this by binding leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), and competing with WNT antagonist DKK1 for binding to the WNT coreceptors, Kremen and LRP6, which reduces DKK1-mediated internalization of LRP6 (Binnerts et al.). RSPO1 is involved in a wide range of pleiotropic roles during embryogenesis, it is required for the specification of hematopoietic stem cells, and it has been shown to be important in the growth, survival, and migration of ovarian cancer cells
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