Human embryonic kidney 293 cells, HEK293
Gln28-Cys232, with a stable fusion tag at the C-terminal
Predicted Molecular Mass
The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) .
Measured by its ability to bind BMP2 with Elisa method
Optimal concentrations should be determined by each laboratory for each application.
<1 EU per 1 μg of the protein by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from a 0.2 μm filtered solution in PBS.
- PREPARATION AND STORAGE
The product is shipped with dry ice.
For long term storage, the product should be stored at lyophilized state at -80°C，avoid repeated freezethaw cycles.
>12 months,under -80°C conditions
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion