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规格单价
100ug3000

Recombinant Human Wnt-3a

Catalog number: WN3-H100F

DESCRIPTION
Source

Human embryonic kidney 293 cells, HEK293
Ser19­Lys352, with a fusion tag at the C-terminal
UniProtKB# P56704

Species

Human

Predicted Molecular Mass

63.3 kDa

SPECIFICATIONS
SDS-PAGE

The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Activity

Measured by its ability to bind wnt3a antibody with elisa method
Optimal concentrations should be determined by each laboratory for each application.

Endotoxin Level

<1 EU per 1 μg of the protein by the LAL method.

Purity

>75% as determined by SDS-PAGE.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS.

PREPARATION AND STORAGE
Shipping

The product is shipped with dry ice.

Storage

For long term storage, the product should be stored at lyophilized state at -80°C,avoid repeated freeze­thaw cycles.

Stability

>6 months,under -80°C conditions

DATA

BACKGROUND
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes . Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels

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